Part:BBa_R0074

Promoter (PenI regulated)

PenP operator from Bacillus Lichenformis. Two operator sites are negatively regulated by PenI (C0074).

Expression Level of the Mnt- and PenI-Regulated Promoters

(Characterized by SDU-Denmark)
The PenI-regulated promoter exhibits a stronger expression than the Mnt-regulated promoter.
Applicability of two different promoters was studied for the expression of genes in E. coli. These were the PenI-regulated, BBa_R0074, and the Mnt-regulated promoters, BBa_R0073, whose repressors are not found in E. coli, making the gene expression constitutive in this organism. The relative expression and noise levels were assessed using fluorescence microscopy of YFP reporter systems, BBa_I6103 and BBa_I6104, expressed on pSB1C3 in MG1655 at OD₆₀₀=0.3-0.5. For this purpose an Olympus IX83 with a photometrics prime camera was used with exposure time for YFP at 200 ms.

Figure 1. Fluorescence microscopy of YFP under the control of PenI-regulated and Mnt-regulated promoters on pSB1C3 in E. coli MG1655 at OD₆₀₀=0.3-0.5.

From the data obtained, it was evident that the PenI-regulated promoter mediated a substantially higher level of YFP expression than the Mnt-regulated promoter, as seen in Figure 1. Furthermore, the PenI-regulated promoter displayed a notably lower level of noise.

Usage and Biology

Sequence is taken from Figure 3 of Himeno et al., J. Bact. 168:1128 (1986). Sequence is extended to -50 (conservative truncation during harvesting)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]